FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50968122
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG588550.11547413891373122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187450.015.32461345
GGCATAG190400.015.23755835
ATAGGGG195900.014.91271938
GGGAGGT295500.013.879283
CATAGGG211900.013.73359237
TGGCATA217400.013.45886734
GAGCTTA217150.013.31114344
GGAGGTC300900.013.1450094
GTCACTC290400.013.1339348
ACTCTCC300350.013.01387811
GCATAGG224600.012.92673536
GGTCACT296400.012.8509987
AGGTCAC346600.011.4941826
CGTCGAT24150.011.36963312
GAGGTCA363200.011.1269445
TAGGGGA344100.010.97516539
CACTCTC358950.010.93638910
TCAGACG85000.010.7282962
TAGCGTA9250.010.70600737
GCGTCGA26050.010.53869111
Produced by FastQC (version 0.11.5)