FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44531761
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133500.013.23029545
ATAGGGG145150.012.19978138
GGCATAG144850.012.05389235
GTTGTCG23750.011.5575226
GGGAGGT232800.011.2697253
CATAGGG163300.010.8711637
GAGCTTA163750.010.73144544
GTCACTC220300.010.6007888
GCATAGG169400.010.4132636
GGAGGTC231450.010.35354
TGGCATA172650.010.34756234
ACTCTCC228150.010.21628811
GGTCACT232350.09.973357
ACCCGGC62300.09.4261335
TAGGGGA261750.08.86229739
GAGGTCA282800.08.7358275
AGGTCAC279900.08.721196
TCAGACG67050.08.5915422
TGTCGTT34650.08.37626828
CACTCTC272600.08.36885310
Produced by FastQC (version 0.11.5)