FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45821448
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT570530.12451156061240143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG117550.023.0638875
AGACCGC118650.022.8879874
ACCGCGT120950.022.4341456
CAGACCG126450.021.6718773
CCGCGTT130950.020.7381427
CGCGTTC139250.019.5666758
GAACGAT160100.017.01848445
CCGTACG82650.016.6872844
AATACGG168250.016.15429532
TCAGACC175800.016.0617522
TTCGGCG84000.015.9905444
TACGGAG173250.015.77898834
CGTACGC89050.015.7406335
ATACGGA175350.015.61568233
TCGGCGC87400.015.39422445
GTCCGTT40350.015.2433661
ACGCTAC139600.015.18215924
CTCAGAC231850.015.1204361
AACGCTA140700.015.09544823
GCGTTCT182300.015.0077299
Produced by FastQC (version 0.11.5)