FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45821448
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG557000.12155879491193732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG158800.017.63965845
GGCATAG167850.016.54264535
GAGCTTA184100.015.31411244
ATAGGGG185350.015.26067438
GGGAGGT290600.015.0379933
GGTCACT279650.014.7069067
TAGCGTA8600.014.65238337
TGGCATA194050.014.50623834
GTCACTC292750.014.0872278
AGGTCAC301400.013.9736316
GAGGTCA304850.013.9708845
ACTCTCC300150.013.89732811
GGAGGTC312100.013.5599264
GTTGTCG24100.013.53501626
CATAGGG214350.013.10116237
TCACTCT329200.012.7461479
CACTCTC333950.012.43010610
GCATAGG225700.012.41225936
TAGGGGA336400.012.09407139
CGTCGAT24950.012.08295912
Produced by FastQC (version 0.11.5)