FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52133752
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4090260.7845704257004177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG132700.016.5509743
AGCTTAG162650.016.33978745
ACCCGGC133100.016.3302695
GGCATAG168200.015.54373835
ACGGACA144350.014.60456218
CCGGACA147150.014.57240112
CGGACAC149850.014.29515113
CACCCGG157700.014.0678224
GAGCTTA194550.013.79937444
CGGACAG152850.013.74828119
TGGCATA200800.013.22181634
CATAGGG200150.013.21870337
GCCCGGA166800.013.09850210
GGTCACT277850.013.0295747
GTCACTC281250.012.9838618
GCATAGG207150.012.72984436
ATAGGGG209650.012.68399138
ATAGCTC164250.012.61508339
ACACGGA169250.012.53718416
AGGTCAC295950.012.5139746
Produced by FastQC (version 0.11.5)