FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50315523
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA582520.1157734164862005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33100.015.08911626
AGCTTAG171050.014.8621845
ATAGGGG181550.014.03983838
GGCATAG180550.013.81848335
GGGAGGT277150.013.1499523
GTCACTC258200.012.8023048
GAGCTTA203750.012.68672844
CATAGGG197900.012.67529237
GGAGGTC273200.012.6061444
GCATAGG205800.012.23238336
ACTCTCC272750.011.99552311
TGGCATA214450.011.81241334
GGTCACT289200.011.5007547
TGTCGTT46150.011.1635928
AGGGATC138900.010.68092
TAGGGGA310400.010.55285239
ATATAGC59200.010.3779686
GAGGTCA343700.010.3012125
CACTCTC320600.010.26841410
GTCGTTC50750.010.2403529
Produced by FastQC (version 0.11.5)