FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48513004
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG95250.09.7340681
ACCCGGG88750.09.4327632
GCGTAGG84850.09.27867139
GGCGTAG86300.09.17491238
CGTAGGG86500.09.15368940
TAATCGG41200.09.0675244
GTTGTCG24250.08.7210126
TGGCGTA95750.08.43384637
CACCGAA110150.08.29109945
GCACCGA111500.08.2310744
GTAACGA41850.08.22673818
ATCGGTT50650.08.2200866
CGTAACG10950.08.21703342
ATAATCG50450.08.0294133
CTAGAAT80600.07.90157271
TCAGACG67700.07.8116642
ACCCGGC62400.07.7542095
GTAGGGG105450.07.67936141
AATCGGT51700.07.53076655
GGTAACG46500.07.35586917
Produced by FastQC (version 0.11.5)