FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54086534
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG164950.015.28338945
ATAGGGG180700.014.17040538
GGCATAG178350.014.05265635
GAGCTTA197800.012.95000644
GTCACTC261350.012.7838948
CATAGGG198350.012.63705637
GGGAGGT295300.012.4177013
GTTGTCG34650.012.27202326
GCATAGG207250.012.20259636
GGTCACT274300.012.0808427
TGGCATA213200.011.87195134
GGAGGTC303150.011.6063654
AGGTCAC298800.011.5645746
ACTCTCC294900.011.55953711
GAGGTCA328900.010.7866875
TGTCGTT42750.010.157330528
TAGGGGA329000.010.01965439
ATATAGC52700.09.6475466
TCACTCT361250.09.5546969
GTCGTTC49250.09.27327929
Produced by FastQC (version 0.11.5)