FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46334809
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA777230.16774213960825865No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG702280.15156639579543751No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG572650.123589588984817No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN508970.10984614180669225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG215800.016.85681745
GGCATAG222300.016.32240535
GTCACTC280500.016.024948
ATAGGGG228300.015.98135238
GGTCACT290800.015.5635357
ACTCTCC302650.015.03113911
GAGCTTA246200.014.91248544
TGGCATA250300.014.70314634
GGAGGTC319450.014.6440664
CATAGGG250100.014.58765537
GGGAGGT335100.014.5080993
AGGTCAC327650.014.2562936
GAGGTCA335050.014.0708325
GCATAGG263800.013.83117836
TCACTCT336350.013.5981769
CACTCTC346150.013.06363410
GTTGTCG28650.012.01373826
AGGGATC144450.011.9452382
AACTCTA92550.011.74052945
TGGGAGG335150.011.5721762
Produced by FastQC (version 0.11.5)