FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49716287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA11600.014.16102537
TCAGACG162950.013.5872242
ATAGCGT13900.011.65594636
AGCTTAG157700.011.58261845
GTCACTC219400.010.9375528
GGCATAG166350.010.902935
GGTCACT215000.010.8385657
CGTGGCG328650.010.5981471
CAGACGT209250.010.5700753
GGGAGGT245200.010.5344393
GCGTATA16650.010.40514539
ATAGGGG177950.010.34350238
ACTCTCC234400.010.34244811
ACCCGGC87100.010.2535965
GTGGCGA340750.010.2481592
ATAGCTC82200.010.12748239
AGACGTG224750.010.000484
TGCCCGT205100.09.91495844
TGGCGAC359150.09.8484223
TAGGCTA80100.09.79839910
Produced by FastQC (version 0.11.5)