FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53299685
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1820060.34147668977781015No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG860500.16144560704251817No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA669210.12555608912135222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC362500.024.8055114
CAGACCG367400.024.5787493
GACCGCG372100.024.1413615
ACCGCGT381650.023.5078096
TCAGACC413950.022.075592
CTCAGAC492700.019.6207471
CCGCGTT466450.019.2389417
CGCGTTC492250.018.2397218
GCGTTCT544300.016.5409569
TTCGACG21450.015.5184326
TTCGGCG116000.015.00567244
CGTTCTC609650.015.00030810
TCGGCGC123750.014.08941545
ACGCTAC128350.014.03753324
AACGCTA128850.013.91301123
ACGTTCG18750.013.4347676
CGTACGC132650.013.3438425
CCGTACG133100.013.3156254
CTCGGTT90900.013.2688951
CGCTACC138550.012.90702525
Produced by FastQC (version 0.11.5)