FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50161174
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG90600.015.92002417
AGCGCTC90550.015.5985199
GGCATAG139950.014.50366935
TCTCGGG104300.014.10703514
AGCTTAG144750.014.03595145
ACGTCTC104700.013.86420621
CAGCGCT103100.013.6997268
ATAGGGG158150.013.16130538
TCGGGAC113500.012.94532516
GACGTCT115700.012.93579820
CATAGGG159000.012.92146637
CGCTCTC118000.012.67530311
GGGACGT118700.012.41754318
GGGAGGT278850.012.3639523
GAGCTTA168850.012.21933244
ACCCGGC82900.012.1821385
TGGCATA168200.012.17450734
GGAGGTC265250.011.9383614
GCATAGG171650.011.90367536
CGTAGGG85100.011.872848537
Produced by FastQC (version 0.11.5)