FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54965120
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA152150.013.05759145
GTTGTCG33600.011.24874326
TATATAC77500.011.0323617
GCGTCGT39150.010.97665311
GGTCGCC43400.010.93972943
CACCGAA239700.010.31582545
TAATCGG59050.010.0598414
TCGTGTA41550.09.962749514
GCACCGA248250.09.92492544
CGTGGCG141450.09.8671351
TCAGACG95600.09.46377852
CATGCAT115300.09.308269
AATCGGT72350.09.1745985
GTAACGA60500.09.11051218
GTCGTGT47350.08.98003913
GGCGACC157950.08.8893924
ATCGGTT69650.08.8842236
TCTATAG287050.08.8580613
AGGGATC129650.08.8009972
ATAATCG69550.08.7677863
Produced by FastQC (version 0.11.5)