FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54822825
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1593930.290742040381903No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG973140.17750635798137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG57750.029.1319736
TCGACGA63750.026.742957
CGACGAG63250.025.923128
CGGTATA90100.023.64017320
GTTCGAC72600.023.0417695
GACCGCG366950.022.7015825
ACCGCGT369400.022.6011926
AGACCGC373800.022.5026954
GAACGAT375300.021.9254245
CCGCGTT381950.021.852687
CAGACCG387950.021.8272193
CGCGTTC384000.021.7653068
TCAGACC433500.019.9394662
GCGTTCT437500.019.1859749
ATGACGG110250.018.64609516
CTCAGAC505450.018.6422461
CAGTTCG97750.018.6355923
AGTTCGA95000.018.3207074
TACGGAG457950.017.85959634
AGAACGA462350.017.8538144
Produced by FastQC (version 0.11.5)