FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43858026
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG469970.10715712558517795No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC464180.10583695672942509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA93700.017.5310723
ACGCTAC94600.017.36513524
AACGATC142400.017.27040921
TACGGAG143500.017.0881989
AATACGG143300.017.064937
TTCGGCG41800.017.06145344
GAACGAT141950.017.05446620
ATACGGA147550.016.847848
GTCGGTT38600.016.8055211
CGCTACC98600.016.63909725
CCCGTAT30800.016.5274011
GTTCCTA114400.016.46041915
GTCCGTT35650.016.3007281
TCGGCGC45050.015.7307145
CCTACTC120800.015.68144618
GACGGCA155450.015.61546138
GTCCGAT22750.015.5440821
AGACGCT220950.015.32387541
ACGATCA163550.015.25739522
AGAACGA158550.015.25457319
Produced by FastQC (version 0.11.5)