FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43858026
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20250.013.22249426
CCGACAA152050.013.09366240
GCCGACA153400.013.03719539
AGCTTAG107800.013.02150545
ACCCGGC64750.012.7882285
GGCATAG112500.012.29890635
ATAGGGG120150.012.17056438
CATAGGG122000.011.41453937
ACAGACT200650.011.2926822
GCATAGG126700.011.09796536
GCGTCGT18600.011.00960811
AGACTCA219450.010.9711964
CGACAAG184750.010.9344341
GAGCTTA134000.010.79447644
TGCCGAC191650.010.75245938
GCACCGA161450.010.57545644
CACCGAA165100.010.43703445
CGGACAC76250.010.35884213
CAGACTC226400.010.3561443
ACGGACA81750.010.32230118
Produced by FastQC (version 0.11.5)