FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43040195
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT38900.018.41331
GTCGGTT47050.017.4737991
TTCGACG21500.017.3716326
CTCGGTT113850.017.1530041
CCCGTAT29050.016.4378051
GCGGACT43200.016.4058152
TCGACGA24700.016.2141447
GTCCGCT40550.016.0531791
GTCCGAT22000.015.8694951
GTCCGGT30050.015.44105151
GCCCGTT43900.015.1873251
CTCCGTT58300.015.0291751
AACGCTA88200.015.02512923
TCGGTTT137650.014.9560152
TACGTGC43800.014.94823533
CCCCCCT30150.014.7921721
CGGTTTT130000.014.7803763
ACGAACG40550.014.75916431
ACGCTAC90250.014.65890824
CGAACGA41300.014.38218532
Produced by FastQC (version 0.11.5)