FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50247806
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT614040.12220235048670583No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT515540.10259950454354165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29600.023.1782556
ACCGCGT156100.022.3069766
GACCGCG158600.021.8576775
AGACCGC161000.021.7134954
CCGCGTT167300.020.813627
CGCGTTC172950.020.1467388
CAGACCG182050.019.4993843
TCGACGA36800.019.1323857
CGGTATA48250.018.508620
TCAGACC216350.017.0110452
CGACGAG38900.016.711768
GTTCGAC39650.016.3400695
GCGTTCT217700.016.1190749
ATACGGA210350.015.47821733
AATACGG229150.015.34723632
AACGCTA244050.015.31948823
ACGCTAC245000.015.24176824
GTTAGTT172250.015.21245344
TCGGCGC82300.014.8765645
GAACGAT236100.014.78492945
Produced by FastQC (version 0.11.5)