FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50247806
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181500.013.50266345
GTTGTCG35650.012.74787626
GGGAGGT286450.012.4184173
GGCATAG199050.012.26555235
GGAGGTC282400.012.0860324
ATAGGGG206600.012.078258538
GTCACTC269800.012.0479598
GGTCACT282700.011.5848927
TCAGACG120850.011.5825552
GAGCTTA216250.011.49656744
AGGTCAC297300.011.4471986
ACTCTCC297850.011.15447911
GAGGTCA311750.011.1500135
ATATAGC49900.010.818476
TGGCATA232250.010.73503834
TGTCGTT45750.010.6221128
CATAGGG232450.010.60871837
TATAGCT52800.010.3521087
GCATAGG242350.010.18509236
CAGACGT136150.09.5354543
Produced by FastQC (version 0.11.5)