FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54058762
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG841200.15560844697109416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN675690.12499176359236638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT391850.015.2931233
AGCTTAG278950.014.59353145
GGTCACT389300.014.5366997
GGAGGTC406100.014.5124954
GTCACTC395800.014.46342858
GGCATAG285850.014.40508935
ACTCTCC398000.014.33290911
AGGTCAC414300.014.0068846
GAGGTCA425850.013.8502855
ATAGGGG306450.013.59137938
TGGCATA313250.013.25298134
TCAGACG124500.013.2071322
TAGCGTA10350.012.82671737
GAGCTTA322750.012.72453744
CATAGGG325900.012.71771637
TGGGAGG346750.012.5393742
CACTCTC459400.012.46146110
TCACTCT477000.012.2274819
GCATAGG341250.012.11961536
TCTCCCC492300.011.77642613
Produced by FastQC (version 0.11.5)