FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49584167
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT8444731.7031101883792865No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG6037341.2175943179604087No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC3820000.7704072148676008No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT3158530.6370037435538647No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT2519380.5081017091605068No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC2216730.44706407995116665No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCT2091680.42184433591472864No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC1889690.38110754184899387No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG1876540.3784554855988606No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1389610.280252766977007No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG1110420.22394648678881707No Hit
GGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA1109810.22382346364717592No Hit
CTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAA1085550.21893077280092252No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG1050830.21192853759144525No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1009790.20365170196365306No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC982080.19806322449664227No Hit
TGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTC918330.185206297808734No Hit
CGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA861570.17375909531766462No Hit
GCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAATAT704400.14206147700333457No Hit
GGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGG659070.1329194458384266No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAAT652670.1316287112376013No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAATA652310.13155610741630488No Hit
CTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCTCCGCTGACTAA630740.12720592845696085No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA601440.12129678411255754No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAC587230.11843094994416262No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG580680.11710996375113047No Hit
CCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAATATG556510.11223542386020118No Hit
GCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGA501490.10113913983873118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT789750.036.909841
CCGGCTT1004850.031.089732
TCACGCT1012700.030.9209188
CGGCTTC1052300.030.1739643
CACGCTC1059600.029.683919
AACGCTA1058400.029.5497623
ACGCTAC1059300.029.48643124
CGCTACC1070600.029.12688425
AAAACGC1075800.029.05715821
AAACGCT1079950.028.97468622
GAAAACG1080550.028.8628120
GGCTTCA1143400.027.9918964
ACGCTCA1152000.027.50217610
TTCACGC1156150.027.2341987
GTTTTCG1119800.027.11142245
CGTGGTT1148550.026.98354541
TCGTGGT1156900.026.7946240
CTTCACG1183000.026.6350866
GCTCAGG1179300.026.51265312
CTACCTC1189500.026.3950427
Produced by FastQC (version 0.11.5)