FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49584167
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT3598230.7256812441761904No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC3303380.6662166977616061No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT2786770.5620281974284251No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT2576310.5195831967894107No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC2049870.41341220878027457No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAA2038600.41113930581913377No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGA1981310.3995852143689335No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT1706860.34423488449447986No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA1682190.3392595059628611No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAA1442930.2910061996201328No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA1430880.28857598837951637No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAA1257080.25352447687585433No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1242350.25055377052114236No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA1072920.21638358873710636No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1008970.20348632659292232No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCA965230.1946649623054069No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG943710.1903248672101318No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCA940030.18958269481465728No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAA931500.1878623876044948No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAG880830.17764339975702323No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC865550.17456177089755284No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA796120.16055931725141212No Hit
AGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAAC762560.15379102768833447No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA752990.1518609761055379No Hit
TTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAG747980.15085057292582935No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA663280.13376850719303202No Hit
ATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCA641120.1292993386376744No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAA600760.12115964356121986No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC595930.1201855422921595No Hit
GCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACG584640.11790860578539113No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG574700.11590393360848433No Hit
CCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGA546700.11025696972987364No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAG545560.11002705762910164No Hit
CCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT545460.11000688990096374No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA531600.10721164278105146No Hit
TTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAG517450.10435790924953926No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA497400.10031427975789127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAC70200.020.523251
TCAGACG902800.018.7211112
ACCCGGC87350.018.2341425
CAGATCA446100.018.161551
CGGACAC90600.017.6049513
AGATCAG456300.017.5980152
CCGGACA95050.016.78069712
TCACCCG96500.016.599463
ATCAGAC1061150.016.0506571
AACTCTA97600.015.76940345
CACCCGG103850.015.4673514
ACGGACA104000.015.40160718
TTCGCGA4900.015.15466427
ACACGGA107800.015.00468516
GCCCGGA110850.014.79475210
TAATCGG48000.014.6699064
CGGACAG109750.014.43109719
CACGGAC108500.014.38952617
GATCAGA569650.014.0559823
GACACGG114150.013.99262615
Produced by FastQC (version 0.11.5)