FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50195737
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG529840.10555478047866894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA155250.017.98274423
ACGCTAC155350.017.97224224
TTCGACG21300.017.5301786
CGCTACC160950.017.37488225
TCACGCT180450.016.3909028
AAAACGC180100.015.67408521
CCGGCTT210400.015.57811452
TTCACGC194500.015.3348057
ACCGCGT74300.015.2883416
AGACCGC76050.014.9662344
GCCGGCT208950.014.9344271
GACCGCG76900.014.7423085
GTCCGTT43550.014.6824261
GAAAACG195900.014.4328820
GTTAGTT131450.014.33537844
CCCGTAT31950.014.2346961
AAACGCT199800.014.13071122
CTACACG55200.014.08541645
ACGCTCA224450.013.91927610
CGGCTTC245450.013.8482293
Produced by FastQC (version 0.11.5)