FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50195737
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG164150.014.84085945
ATAGGGG180000.013.64171838
GGCATAG176850.013.61238135
GTTGTCG36950.012.66526326
GGGAGGT283250.012.5918893
GAGCTTA199550.012.4805244
GTCACTC270400.012.0741688
GGAGGTC280700.012.0562524
CATAGGG202100.012.01633337
GGTCACT278950.011.6960797
ACTCTCC279500.011.68789511
GCATAGG213700.011.4128736
TGGCATA217850.011.30827434
ATATAGC49800.011.2954766
GAGGTCA304800.011.2136545
AGGTCAC310000.010.9672226
TAGGGGA310200.010.50614939
TATAGCT57350.010.3186047
TGTCGTT48500.010.02072128
TCACTCT343800.09.5742099
Produced by FastQC (version 0.11.5)