FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47685263
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG41600.027.5275426
TCGACGA46850.024.9216657
CGACGAG48000.023.7152738
CGGTATA68300.022.07058720
GTTCGAC58300.019.4881365
ATGACGG86300.016.97081416
CCCGTAT29200.016.5848881
AACGACG26250.016.28383816
ACCGCGT34250.016.1591136
GTATAGA101800.016.15727622
CAGTTCG78650.016.0190873
GTCGGTT45450.015.9580181
ACGAGGA93250.015.48834610
GTCCGTT41300.015.4345271
AGTTCGA77600.015.3660624
GACCGCG36750.014.9987935
AACGCTA98100.014.771667523
GTTCCTA86100.014.63226215
ATAGAGT117300.014.27292924
ACGCTAC101900.014.22164424
Produced by FastQC (version 0.11.5)