FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53302095
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT988680.1854861427116514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC233950.021.4901284
ACCGCGT234300.021.4100236
GACCGCG233900.021.3889315
CCGCGTT250050.020.07047
CAGACCG252550.020.0493183
CGCGTTC255300.019.7017638
GAACGAT261050.019.3180345
TCAGACC303500.017.2097682
GCGTTCT304550.016.6487129
AGAACGA306200.016.52567944
TACGGAG313550.015.92671234
CTCAGAC361400.015.877351
AATACGG318600.015.7634332
ATACGGA320100.015.68959133
TTCGACG17500.015.4250916
CCCGTAT37050.015.3779741
AACGCTA93950.015.34834923
ACGCTAC94650.015.32990724
ACGGAGA341100.014.73337435
CAATACG343850.014.68292231
Produced by FastQC (version 0.11.5)