FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53302095
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122000.011.3911445
GGCATAG121650.011.13563235
ATAGGGG130750.010.8391938
GTTGTCG30150.010.67189926
ACCCGGC81750.010.2105935
CATAGGG137850.010.02020637
GTCACTC199100.09.5347748
GCATAGG145600.09.47275336
ACGGACA91050.09.41476418
GGTCACT200350.09.3959767
GAGCTTA152000.09.26412144
ATAGCTC88850.09.03875739
GGGAGGT263550.08.896053
ACTCTCC221000.08.80357311
TGGCATA155250.08.79807334
TCACCCG107250.08.7694343
TGTCGTT38600.08.68519728
CGGACAC102150.08.65626313
CCGGACA107600.08.57247512
GGAGGTC240550.08.3903344
Produced by FastQC (version 0.11.5)