FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46381003
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA827380.17838769032226406No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT737880.15909099680315236No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG622630.13424246129390519No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC543720.11722903016995988No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTG469080.10113623459156328No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGA468010.10090553669139066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA2350.028.72083535
TTCACGC207750.025.1020687
TTCGACG22100.024.7372346
TCACGCT212600.024.5400038
GCGGACT132300.024.2030282
ACGCTAC206800.023.89342324
AACGCTA207150.023.83117523
CGCTACC211300.023.35292625
CCGGCTT151400.022.6062242
CGGACTT150900.022.4276243
GCCGGCT147600.022.0232831
CACGCTC235750.021.968019
AAAACGC233000.021.43682121
GGCGGAC177000.020.5302831
AAACGCT242750.020.4279422
GAAAACG251300.020.17992820
TACGCGC1603.8198777E-1119.6856317
TACCTCT259650.019.33390228
GCTACCT265700.019.15593126
CGTACCG2500.018.9018363
Produced by FastQC (version 0.11.5)