FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46381003
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002580.2161617764066034No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC709230.15291389882189482No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC565670.12196157120621132No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCT557040.12010089561883773No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG525930.11339340807269736No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC525660.11333519458386875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG4250.020.12025534
CGCGTAA1302.7786882E-819.0388743
ACGTAGC19950.018.7155426
CGTAGCC18250.018.3638957
CGCTTAA16200.017.7779916
GCGCTTA16950.016.59266915
ACGTCGA6700.016.11077711
GTACGCG11300.015.93513829
GGGAGGT226650.015.6654623
CGTAATA9200.015.65790332
GTTGTCG30550.015.471992526
TATCGCG4650.015.0003243
GGAGGTC240450.014.6996424
CGTAGCG6750.014.66856734
TATACGC10300.014.63528941
CGCGAAC3100.014.51705322
CGGCGTA4350.014.4829316
CGTTCGG9950.014.47354819
CGGTAAT10700.014.2993892
ATAGCGT6150.014.27000336
Produced by FastQC (version 0.11.5)