FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46309931
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG474120.10237976817542656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG162400.014.68325745
ATAGGGG164500.014.55451738
GGCATAG162100.014.41096735
GTTGTCG34650.013.51783926
GTCACTC239050.013.265478
GGTCACT236550.013.2448977
GAGCTTA181850.013.11432744
ATATAGC43450.012.7162786
AGGTCAC254200.012.6261626
CATAGGG188700.012.58085637
TGGCATA192550.012.2388634
GGGAGGT279900.012.2301683
ACTCTCC266600.011.91970311
GAGGTCA276000.011.8002575
TGTCGTT40200.011.79153128
GGAGGTC277450.011.7550314
GCATAGG207000.011.48069836
TCACTCT316750.010.1247649
TAGGGGA312200.010.01541739
TATAGCT56250.09.6225877
Produced by FastQC (version 0.11.5)