FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46359821
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22400.018.9842956
GTCCGTT37750.018.6723651
CCCGTAT34750.017.3680861
CTCGGTT102500.016.346361
ATGCGCG18150.016.2392544
TCGACGA26950.016.1963487
CTCCGAT49300.016.1706831
CCCGGAT98250.015.6781761
CTCCGTT71550.015.6114791
GCACTAC65350.015.59645325
CTCCTGT425100.015.4372671
CTCCGGT77650.015.4001541
GTCCGGT38050.015.2107441
TCAATCG53050.015.1833978
CCCCCCT36500.015.177991
CGACGAG25950.015.1730198
GTTCCTA81100.014.7871115
TGCGCGT20400.014.44815745
CCGTACG44850.014.44814
AGACGCT137750.014.35716141
Produced by FastQC (version 0.11.5)