FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46359821
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG595860.12852940049099845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG170150.015.97050145
ATAGGGG190250.014.63792538
GGCATAG188200.014.4986535
GAGCTTA200700.013.70763944
GGGAGGT266100.013.4552433
GTTGTCG25000.012.958878526
GGAGGTC271200.012.9532864
TGGCATA216100.012.67886334
GTCACTC271850.012.6497748
CATAGGG217300.012.61907537
GGTCACT271700.012.3171337
GAGGTCA289250.012.2543655
ACTCTCC278650.012.14727711
GCATAGG230050.011.90992536
AGGTCAC291550.011.9029846
TCACTCT330600.010.544819
TAGGGGA320200.010.50275939
TGGGAGG277000.010.431492
TAATCGG32650.010.3389114
CACTCTC337700.010.12317810
Produced by FastQC (version 0.11.5)