FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50368955
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG597160.11855715489829002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA215100.023.41278623
ACGCTAC215850.023.33155324
ACCGCGT99650.022.3910146
CGCTACC224700.022.35255825
TTCGACG30800.022.346536
GACCGCG101600.021.8948525
AAAACGC239150.021.22758721
TCACGCT255450.020.7535828
AGACCGC110200.020.4515174
AAACGCT255500.019.89559622
CCGCGTT112000.019.8416757
GAAAACG257000.019.69195420
TTCACGC270950.019.6742697
ACGCTCA267800.019.67051510
CGCGTTC113650.019.57348
CGGTATA47100.019.29300320
GCCGGTT95150.018.8847221
CACGCTC282650.018.7325489
GCCGGCT185900.018.4863951
CGTGGTT282600.018.08843841
Produced by FastQC (version 0.11.5)