FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50323145
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT96200.023.5261366
GACCGCG96150.023.4213815
AGACCGC99150.022.6446464
CCGCGTT103950.021.6855757
CGCGTTC105100.021.4268868
CAGACCG111200.020.4942673
TTCGACG20450.020.2419436
ACGCTAC115600.017.06809424
AACGCTA115800.016.96036123
GAACGAT134400.016.74121745
CGCTACC120000.016.4800425
AATACGG136300.016.2439232
ATACGGA137600.016.04069533
TCAGACC149500.015.90642
TACGGAG140250.015.57806834
CAATACG149800.014.91521431
GCGTTCT152350.014.8405949
CGGTATA37450.014.7177720
AAAACGC137800.014.49744421
CTCAGAC215700.014.3198681
Produced by FastQC (version 0.11.5)