FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41771947
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT33050.017.37861
TTCGACG16200.015.6941296
TCGTATG28250.015.68981845
CCCGTAT28550.015.3841871
ACGCGTC11900.015.31469140
CTCCGTT67350.014.6146991
CTCGGTT75000.014.5355071
CTCCGAT44350.014.4742911
GTCCGCT32850.014.056091
CACGCGT14450.013.85773839
CTCCGGT58600.013.7988511
GTCGGTT36900.013.4289611
GTCCGAT23500.013.3227221
AACGCTA76450.013.15537723
ACGTTCG14750.013.1183816
AAGCGCG13800.013.04315418
CCCCGCA129400.013.023235
AGACGCT138400.012.94038341
CCCGGAT86500.012.8894721
AAGCGGC131650.012.88616828
Produced by FastQC (version 0.11.5)