FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46680191
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT40750.018.956331
TTCGACG24400.018.7190346
CCCGTAT32350.017.3345571
CGACGAG27000.015.6662578
AGACGCT160250.015.40209441
CTCGGTT102350.015.3367251
CTCCGAT53150.015.2964841
GCACTAC72000.015.28096425
CCCGGAT102850.015.2183721
TCGACGA31600.014.8809887
CTCCGTT78750.014.8710111
CTCCGGT65100.014.8410281
GTCGGTT43700.014.7391451
GTCGGAT46950.014.5343211
GCGAAAC25900.014.50734337
CCTACTC81150.014.47283618
CCCAGAT178000.014.2843881
CCCCCCT39600.014.2178041
GTTCCTA85100.014.1449215
CCCCGTT59850.014.0732741
Produced by FastQC (version 0.11.5)