FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46680191
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG117750.014.34703438
AGCTTAG118700.013.98584645
GGCATAG117900.013.89002435
CATAGGG129400.012.95105437
GCATAGG133500.012.33428136
GGGAGGT179950.012.2307993
GAGCTTA148000.011.65779144
GTCACTC177750.011.2306438
TGGCATA157300.010.53965834
ACTCTCC199350.010.37509811
GGAGGTC199450.010.3241964
TAGGGGA196850.010.0446839
GGTCACT201400.09.8447827
GAGGTCA226200.09.5712765
AGGTCAC225650.09.3352926
TAATCGG25100.09.3245714
GTTGTCG22450.08.81897826
TCACTCT235050.08.77057659
CACTCTC231350.08.68710110
CGTAACG11550.08.56945742
Produced by FastQC (version 0.11.5)