FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52721127
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161750.015.44298545
ACCCGGC92850.015.3145715
GGCATAG175900.014.29863535
GAGCTTA193700.013.11561944
ACGGACA110300.013.05484418
TCACCCG114850.013.02522853
ATAGGGG200450.012.95164638
ATAGCTC104200.012.86778439
TGGCATA202500.012.56481734
CATAGGG202050.012.4925837
CCGGACA123000.012.02074212
GGGAGGT327900.012.0126663
CGGACAC120300.011.99118813
GCATAGG213700.011.87470336
CGTCGAT21100.011.73224912
CGGACAG120000.011.66140319
GGAGGTC315400.011.5907054
TAGCTCT117500.011.58380840
CACGGAC124950.011.50662317
CACCCGG132050.011.1588934
Produced by FastQC (version 0.11.5)