FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47882591
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1112490.23233705126775617No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG818250.17088674253237465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG40050.027.1845366
ACCGCGT228500.025.2215656
GACCGCG228950.025.1252565
AGACCGC230600.025.0730084
CGGTATA58250.024.13643820
CCGCGTT240900.023.9325587
TCGACGA46850.023.6228797
CAGACCG244850.023.6149713
CGCGTTC241650.023.4766458
GAACGAT245150.023.1416445
CGACGAG48200.022.2612278
TCAGACC275050.021.4637972
AATACGG286050.019.95051232
ATACGGA286950.019.92716433
TACGGAG286850.019.78499834
GCGTTCT289100.019.7012319
AGAACGA296500.019.24760444
CTCAGAC360100.018.772271
CAATACG308300.018.75165231
GTTCGAC57900.018.6890355
Produced by FastQC (version 0.11.5)