FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47882591
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG598010.12489090241586968No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA539680.1127090219491255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG200750.015.15078345
GTTGTCG35800.014.51861626
GGCATAG210950.014.42646335
GTCACTC269850.014.1039958
ATAGGGG220650.014.0773538
GGGAGGT305050.013.69463
GGAGGTC294950.013.4180714
GGTCACT285300.013.38637
ACTCTCC286900.013.31385111
GAGCTTA233750.013.24213544
CATAGGG240350.012.81154237
TGGCATA241750.012.77350934
AGGTCAC309000.012.6791016
GAGGTCA317950.012.4416945
TGTCGTT43350.012.1454628
GCATAGG253800.012.06168936
TATAGCT49700.012.04497057
ATATAGC48900.011.872966
TCACTCT331400.011.7833289
CACTCTC325900.011.69297410
Produced by FastQC (version 0.11.5)