FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49044338
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG618200.12604920877920708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG46100.028.4537286
CGGTATA72200.024.99228920
TCGACGA54700.024.1858337
CGACGAG58700.022.1543758
GTTCGAC62850.020.6558025
GTATAGA106650.018.24837122
ATGACGG94200.017.89007616
AGACCGC112550.017.7916434
ACCGCGT113600.017.4885546
ATAGAGT113300.017.17739524
CGAGGAT92750.016.66568611
CAGACCG121900.016.6300143
GACCGCG120800.016.446195
AGTTCGA85100.015.8897284
ACGAGGA110200.015.74161110
CGCGTTC126800.015.7388918
CCGCGTT126550.015.6989157
GACGGTA116900.015.4550818
CCGGCTT252350.015.3979612
TTCACGC236750.015.27203757
Produced by FastQC (version 0.11.5)