FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49044338
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3934740.8022822124747611No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA516930.10540054593050067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA104950.014.90195545
AGCTTAG142600.014.84947145
GGCATAG150100.014.0306735
ATAGGGG156750.013.67823938
GAGCTTA167300.012.73780244
GTTGTCG29950.012.2441726
CATAGGG177200.011.96014637
GGGAGGT252800.011.8832143
TGGCATA181650.011.61834934
GCATAGG185100.011.51156636
GGAGGTC250350.011.440724
CACCGAA252200.011.03736745
TAGCGTA10250.010.97477137
TCAGACG90200.010.9518332
GGTCACT258250.010.6727717
CGTGGCG142850.010.4299471
GCACCGA265500.010.3234144
TGTCGTT36550.010.217813528
ACTCTCC279900.010.04847511
GTCACTC293000.09.9367648
Produced by FastQC (version 0.11.5)