FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46156598
Sequences flagged as poor quality0
Sequence length51-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT47050.032.8736694-95
ACACGTT151650.029.3923358-59
ACCCCTA22950.029.11149270-71
ACACTAT85700.028.9407386-87
GCCGGCT263250.028.8681011
ACTATAG83550.028.69959888-89
CTACACG155200.028.36628356-57
ACGTTCA158500.028.17937560-61
CGTCTGA288350.027.51820894-95
ACGTCTG316400.027.3637594-95
ACACGTC361500.027.03858494-95
CACACGT395200.026.65504394-95
CACGTCT362650.026.52381794-95
CCCTAAG42900.026.24289752-53
CCGGCTT313800.026.2233092
CTAGTAA101150.025.9679178-79
GCCCTAA35050.025.85301252-53
TACACGT147600.025.79612258-59
ACCGTAC60600.025.67422192-93
TCACGCT314000.025.6411978
Produced by FastQC (version 0.11.5)