FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46156598
Sequences flagged as poor quality0
Sequence length51-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA313450.028.78926794-95
TGTAGGG260950.028.54888294-95
GCGTCGT394000.028.5481394-95
GTAGGGA236350.027.72550294-95
GTCGTGT341700.027.53405294-95
AGCGTCG456850.026.5438794-95
CGTCGTG392100.026.16560294-95
GTGTAGG302850.026.04963794-95
AGAGCGT537500.025.91541794-95
TAGGGAA261600.023.68497594-95
AAGAGCG672950.023.16081494-95
ATCGGAA858150.022.8229994-95
GATCGGA897600.022.58462394-95
AGATCGG970700.022.14725994-95
TCGGAAG838350.021.1240794-95
CACAACG82600.021.05218582-83
AGTGTAG111450.020.03695794-95
CGGAAGA847700.019.61709894-95
GAGCGTC669550.019.25972294-95
CAGATCG394650.018.71472494-95
Produced by FastQC (version 0.11.5)