FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44954686
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC47650.023.32898133
ACGGCTA48400.022.97000732
TCGTATG50650.021.8542443
GTCCGTT35750.019.3412971
AGTCACG62800.017.56016528
ACGTTCG14950.016.8542546
GTATGCC68000.016.8406945
TATGCCG16350.016.78777545
GCCGGCT148150.016.6317881
CCCCGCA151500.016.48364335
CCCGTAT30900.016.473021
AGACGCT161150.016.34810841
CTCGGTT101450.016.0290741
ACACGTC71950.016.00981513
AAGCGGC154750.015.88122928
CCCCCCT29550.015.7012051
TCACGCT161200.015.7007428
CTCCGGT63950.015.6374411
GTCCGAT22550.015.5812151
CGTCTGA71500.015.5441916
Produced by FastQC (version 0.11.5)