FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44954686
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC54550.019.9587443
TCGTGTA61650.017.66710314
GCGTCGT61700.017.4704811
GTCGTGT63200.017.19826113
TCTCGGT67500.016.3968836
GTAGGGA67400.016.39437918
GTCGCCG73500.015.94530744
CGCCGTA16350.015.40929545
TGTAGGG73600.014.98283717
AGTGTAG74750.014.86766228
TGGTCGC89200.014.45014742
CGTCGTG75650.014.27863912
TCGGTGG80150.013.94924938
CGGTGGT81050.013.87761939
ATCTCGG81900.013.62382735
GTAGATC81900.013.56973731
AGCGTCG81950.013.37256410
CTCGGTG85350.013.17845337
ACCCGGC67600.012.8819925
TAGATCT88600.012.56873832
Produced by FastQC (version 0.11.5)