FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54807069
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG913920.16675221220094802No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT686580.1252721615162453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA220050.028.49328823
ACGCTAC222300.028.21509224
CGCTACC230200.027.11975925
TCACGCT246600.026.0915558
AAAACGC245050.025.70575121
GAAAACG255700.024.64389420
AAACGCT265300.023.72670622
ACGCTCA277400.023.4784310
TTCACGC279650.023.1367847
CACGCTC294750.021.85989
GTTTTCG256650.020.73142645
TCGTGGT300900.020.6952640
CGTGGTT304850.020.53042441
TACCTCT334100.019.15728428
CTACCTC339600.018.73440427
TTCGACG25100.018.5534886
ACGTTCG25200.018.4798626
AGAAAAC350350.018.2815219
TTCCTCG354900.017.90778236
TACGGTA86450.017.66980644
Produced by FastQC (version 0.11.5)