FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49183524
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC122750.016.5458325
ACGGACA127700.015.78754318
CGGACAC136200.014.70160813
TCACCCG144950.014.5866013
ATAGCTC134600.014.56143439
CGGACAG138800.014.44374819
CACCCGG147600.013.988234
CCGGACA150100.013.71293512
AGCTTAG114550.013.62170445
ACACGGA146700.013.55939716
TAGCTCT148800.013.12770840
CACGGAC153250.013.09728417
GACACGG153100.013.05078215
GGCATAG121100.012.8777935
TGATAGC156950.012.81651937
GCCCGGA163400.012.7457510
TTGATAG161200.012.49328536
TCAGACG124100.012.0167162
CATAGGG130300.011.88058837
TAGCGTA18200.011.74481437
Produced by FastQC (version 0.11.5)