FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43281059
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG94050.011.3867572
ATAGGGG106800.010.36551438
CCGTACG18300.010.2042564
CGTGGCG162000.09.8270621
GGCATAG106850.09.77096635
AGCTTAG107550.09.74824445
GTTGTCG20200.09.24493126
GTCACTC152250.09.1621178
CAGACGT116800.09.0340473
TGGCGAC179350.08.9316353
GTGGCGA177550.08.8574432
GGCGACC179100.08.79344
AGGGATC116350.08.7789242
CGACCCG182350.08.6738916
GCGACCC185100.08.64205555
CGTACGC21650.08.6252975
GGTCACT161000.08.6223127
GAGCTTA125600.08.544444
GGGAGGT187000.08.4820313
GTAACGG66650.08.43950226
Produced by FastQC (version 0.11.5)