FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49130785
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG148100.013.60171645
GGCATAG147400.013.51701135
ATAGGGG159700.012.90749638
GGGAGGT248550.012.2227443
GTTGTCG24700.012.13558526
CATAGGG166950.012.09420537
GAGCTTA172700.011.81892344
TGGCATA177750.011.49551934
GGAGGTC239600.011.4917154
CGTCGAT23600.011.47965212
GCATAGG176000.011.39655636
GTCACTC239150.011.2914768
GGTCACT238000.011.1023097
ACTCTCC242700.010.96160511
GCGTCGA25900.010.80333611
TGCGTCG26100.010.38017410
ACCCGGC79600.010.209615
AGGGATC108950.010.20362
GAGGTCA286300.09.7488875
TAGGGGA275850.09.57364539
Produced by FastQC (version 0.11.5)